Linkage mapping bovine EST-based SNP PMC

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Here, we have characterized SLC35A3 from the domesticated pig by cDNA cloning and sequencing, tissue expression profiling and chromosomal assignment of the gene. In this heterologous expression system, we analyzed mutated versions of SLC35A3 harboring truncations, internal deletions and substitutions of specific residues to identify important regions of the transporter. The conserved membrane topology of NSTs suggests that our mutational study of porcine SLC35A3 has importance for understanding the structure-function relationships of other family members as well. Excel spreadsheet containing linkage maps of bovine autosomes. Data include marker name, locus, and type; PubMed or other reference, chromosome, position on chromosome, 3-lod confidence interval and comparative position on human sequence.

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